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Part:BBa_K4061090:Design

Designed by: Diya Agrawal   Group: iGEM21_HKUST   (2021-10-15)


eGFP antisense characterisation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1325


Design Notes

Since we ordered the construct through Twist Bioscience, we needed to reduce the complexity of the DNA sequence. By nature if are to synthesise this construct, DNA will have secondary structure since we are joining a sense and an antisense strand in the same fragment. We needed to reduce the complexity on other ends, therefore we used two different terminators and optimised the sequence till it gave low complexity scores.


Source

Biobricks

References